Hotspot

trnK-UUU-rps16

Genome · 4365-5264 · 900 bp

Back to Gardneria

Pi 0.00562
MarkerSeek score 63.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
Genome
length_bp
900
variable_sites
5
pi
0.00562
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1072-1082 bp 1.0 79.3
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1073-1083 bp 1.0 79.3
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1077-1087 bp 1.0 79.3
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1075-1085 bp 1.0 79.3
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 1084-1094 bp 1.0 79.0