Hotspot

ndhD

SSC · 116520-118022 · 1503 bp

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Pi 0.000776

MarkerSeek score 48.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 2
pi: 0.000776
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACAATTTCAAAATCATAGGCGGT	ACGGGTTTTTCTGGTCCAAG	34.8	50.0	57.3	58.3	1577 bp	1.0	48.3
ndhD_p2	ACAATTTCAAAATCATAGGCGGT	CGGGTTTTTCTGGTCCAAGT	34.8	50.0	57.3	58.3	1576 bp	1.0	48.3
ndhD_p3	ACAATTTCAAAATCATAGGCGGT	GACAAAACCCGTGCTCAAAA	34.8	45.0	57.3	57.4	1607 bp	1.0	46.3
ndhD_p4	ACAATTTCAAAATCATAGGCGGT	CACGGGTTTTTCTGGTCCAA	34.8	50.0	57.3	58.6	1578 bp	1.0	46.0
ndhD_p5	ACAATTTCAAAATCATAGGCGGTT	CGGGTTTTTCTGGTCCAAGT	33.3	50.0	58.0	58.3	1576 bp	1.0	45.3