Hotspot

rps19-rpl2

IRb · 85274-85333 · 60 bp

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Pi 0.00833

MarkerSeek score 54.70

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 60
variable_sites: 1
pi: 0.00833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	197-210 bp	1.0	71.9
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	264-277 bp	1.0	71.0
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	55.0	50.0	59.2	57.8	255-268 bp	1.0	70.5
rps19-rpl2_p4	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	55.0	45.0	59.2	58.3	199-212 bp	1.0	70.4
rps19-rpl2_p5	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.6	58.1	196-209 bp	1.0	70.1