Hotspot

petB-petD

LSC · 77439-78312 · 874 bp

Back to Gardenia

Pi 0.0107

MarkerSeek score 41.20

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 14
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CCGCTTCTTACTGCCGTATT	TTATGGATCCATCCCCCGAA	50.0	50.0	58.1	57.9	981 bp	1.0	79.6
petB-petD_p2	CCGCTTCTTACTGCCGTATT	TTTATGGATCCATCCCCCGA	50.0	50.0	58.1	57.9	982 bp	1.0	79.6
petB-petD_p3	AAACAGGGTATTTCTGGCCC	TTATGGATCCATCCCCCGAA	50.0	50.0	57.8	57.9	933 bp	1.0	78.3
petB-petD_p4	AAACAGGGTATTTCTGGCCC	TTTATGGATCCATCCCCCGA	50.0	50.0	57.8	57.9	934 bp	1.0	78.3
petB-petD_p5	CCGCTTCTTACTGCCGTATT	TATGGATCCATCCCCCGAAG	50.0	55.0	58.1	58.3	980 bp	1.0	77.9