Hotspot

ndhC-trnV-UAC

LSC · 51051-52122 · 1072 bp

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Pi 0.0028

MarkerSeek score 51.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 5
pi: 0.0028
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATCGGGGCTAAAATTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1207-1353 bp	1.0	76.2
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1215-1361 bp	1.0	76.0
ndhC-trnV-UAC_p3	CGGGGCTAAAATTCCGGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	1204-1350 bp	1.0	74.5
ndhC-trnV-UAC_p4	AATCGGGGCTAAAATTCCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.3	57.5	1206-1352 bp	1.0	73.5
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	1214-1360 bp	1.0	73.2