Hotspot

trnF-GAA-ndhJ

LSC · 48858-49371 · 514 bp

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Pi 0.00519

MarkerSeek score 34.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 4
pi: 0.00519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	655-656 bp	1.0	79.9
trnF-GAA-ndhJ_p2	GGATGCTACATTGGGACTGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	677-678 bp	1.0	79.7
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	619-620 bp	1.0	79.5
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	690-691 bp	1.0	79.0
trnF-GAA-ndhJ_p5	GGATGCTACATTGGGACTGG	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	712-713 bp	1.0	78.8