Hotspot

psbC-trnS-UGA

LSC · 35974-36199 · 226 bp

Back to Gardenia

Pi 0.0177
MarkerSeek score 38.80
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
226
variable_sites
6
pi
0.0177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCATTTATGGCATGCGGGAA GAGAGATGGCTGAGTGGTTG 45.0 55.0 57.9 58.0 420-421 bp 1.0 80.6
psbC-trnS-UGA_p2 CCTGTCCTTTCAATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 347-348 bp 1.0 80.2
psbC-trnS-UGA_p3 TCATTTATGGCATGCGGGAA ATAGCCCCGGTCTTGAAAAC 45.0 50.0 57.9 57.9 400-401 bp 1.0 79.9
psbC-trnS-UGA_p4 CCTGTCCTTTCAATGACCCC ATAGCCCCGGTCTTGAAAAC 55.0 50.0 58.2 57.9 327-328 bp 1.0 79.4
psbC-trnS-UGA_p5 TCATTTATGGCATGCGGGAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.9 58.2 351-352 bp 1.0 79.3