Hotspot

psbk-psbI

LSC · 8219-8628 · 410 bp

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Pi 0.00488

MarkerSeek score 41.40

Primer available yes

Label psbk-psbI

Feature Metadata

label_name: psbk-psbI
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 3
pi: 0.00488
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbk-psbI_p1	CTTTTGTTTGGCAAGCTGCT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.1	57.4	467-468 bp	1.0	59.7
psbk-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	57.9	57.4	462-463 bp	1.0	59.7
psbk-psbI_p3	TGGCAAGCTGCTGTAAGTTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	459-460 bp	1.0	57.7
psbk-psbI_p4	TTGGCAAGCTGCTGTAAGTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	460-461 bp	1.0	57.7
psbk-psbI_p5	TTTGGCAAGCTGCTGTAAGT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	461-462 bp	1.0	57.7