Hotspot

rps16

LSC · 5331-6454 · 1124 bp

Back to Garcinia

Pi 0.0252

MarkerSeek score 67.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1124
variable_sites: 106
pi: 0.0252
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAATTGAGTCGCACGCTACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.2	58.1	2152-2373 bp	0.789474	79.8
rps16_p2	AAATTGAGTCGCACGCTACA	CGGCGGAAATTGACGAAAAA	45.0	45.0	58.2	58.0	2282-2478 bp	0.684211	73.9
rps16_p3	ATTTCCGTAAAAATCCGCGC	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1604-1665 bp	0.526316	66.6
rps16_p4	ATTTCCGTAAAAATCCGCGC	TCCCAGAGCATACCGAGAAT	45.0	50.0	57.8	57.9	1586-1647 bp	0.526316	66.6
rps16_p5	ATTTCCGTAAAAATCCGCGC	CGGCGGAAATTGACGAAAAA	45.0	45.0	57.8	58.0	1717-1778 bp	0.263158	54.4