Hotspot

ndhF-trnL-UAG

SSC · 114910-115521 · 612 bp

Back to Garcinia

Pi 0.0289
MarkerSeek score 69.20
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
612
variable_sites
61
pi
0.0289
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 AGGTATGATCCATGAATATTGA AGCGTGTCTACCGATTTCAC 31.8 50.0 52.1 58.0 666-1345 bp 1.0 48.7
ndhF-trnL-UAG_p2 AGGTATGATCCATGAATATTGA CAGCGTGTCTACCGATTTCA 31.8 50.0 52.1 58.0 667-1346 bp 1.0 48.7
ndhF-trnL-UAG_p3 AAGGTATGATCCATGAATATTGA AGCGTGTCTACCGATTTCAC 30.4 50.0 53.0 58.0 667-1346 bp 1.0 48.7
ndhF-trnL-UAG_p4 AAGGTATGATCCATGAATATTGA CAGCGTGTCTACCGATTTCA 30.4 50.0 53.0 58.0 668-1347 bp 1.0 48.7
ndhF-trnL-UAG_p5 AGGTATGATCCATGAATATTGA TCCTAAGAGCAGCGTGTCTA 31.8 50.0 52.1 57.9 676-1355 bp 1.0 48.7