Hotspot

rpl22-rps19

LSC · 85419-85526 · 108 bp

Back to Garcinia

Pi 0.0724

MarkerSeek score 66.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 27
pi: 0.0724
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	AGAAAATTCCGTCCTCGAGC	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.0	58.0	1040-1138 bp	0.947368	83.6
rpl22-rps19_p2	AGAAAATTCCGTCCTCGAGC	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.0	58.0	1039-1137 bp	0.947368	83.6
rpl22-rps19_p3	AGAAAATTCCGTCCTCGAGC	GACAAGTAGGGAATGCTGGG	50.0	55.0	58.0	58.0	1032-1130 bp	0.947368	83.3
rpl22-rps19_p4	AGAAAATTCCGTCCTCGAGC	TTCTTCGTCGCCGTAGAAAG	50.0	50.0	58.0	58.0	801-899 bp	0.894737	82.3
rpl22-rps19_p5	AGAAAATTCCGTCCTCGAGC	TACCTTCTGGGGAAGTTCGT	50.0	50.0	58.0	58.0	1083-1181 bp	0.894737	81.1