Hotspot

rps8-rpl14

LSC · 81688-81998 · 311 bp

Back to Garcinia

Pi 0.038

MarkerSeek score 67.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 311
variable_sites: 43
pi: 0.038
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCTTACCCA	GGTCCAATTGCTCGGGAATT	45.0	50.0	55.8	58.5	359-431 bp	1.0	70.5
rps8-rpl14_p2	TCTATCCATGTCAGCATTTCGT	GGTCCAATTGCTCGGGAATT	40.9	50.0	57.6	58.5	398-470 bp	1.0	68.5
rps8-rpl14_p3	AGCAATAGTGTCCTTACCCAT	GGTCCAATTGCTCGGGAATT	42.9	50.0	56.4	58.5	359-431 bp	1.0	67.0
rps8-rpl14_p4	AGCAATAGTGTCCTTACCCA	TTTTGGTCCAATTGCTCGGG	45.0	50.0	55.8	59.0	363-435 bp	0.947368	64.3
rps8-rpl14_p5	AGCAATAGTGTCCTTACCCA	TTTTTGGTCCAATTGCTCGG	45.0	45.0	55.8	57.2	364-436 bp	0.894737	63.9