Hotspot

trnP-UGG-psaJ

LSC · 67522-68022 · 501 bp

Back to Garcinia

Pi 0.0224

MarkerSeek score 66.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 41
pi: 0.0224
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	571-603 bp	1.0	88.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	570-602 bp	1.0	88.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	582-614 bp	1.0	88.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	581-613 bp	1.0	88.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	584-616 bp	1.0	88.5