Hotspot

rbcL-accD

LSC · 57130-58126 · 997 bp

Back to Garcinia

Pi 0.0267

MarkerSeek score 62.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 98
pi: 0.0267
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	CCTACACCCGCATTCTAACC	50.0	55.0	58.0	58.1	1048-1238 bp	1.0	91.2
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	TAGCCTACACCCGCATTCTA	50.0	50.0	58.0	57.9	1051-1241 bp	1.0	91.0
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	CCTACACCCGCATTCTAACC	55.0	55.0	58.0	58.1	1003-1193 bp	0.947368	88.3
rbcL-accD_p4	GCTTGCGAAGTATGGAAGGA	CCTACACCCGCATTCTAACC	50.0	55.0	58.0	58.1	970-1126 bp	0.736842	80.3
rbcL-accD_p5	GCTTGCGAAGTATGGAAGGA	TAGCCTACACCCGCATTCTA	50.0	50.0	58.0	57.9	973-1129 bp	0.736842	80.0