Hotspot

ndhC-trnV-UAC

LSC · 51320-51912 · 593 bp

Back to Garcinia

Pi 0.0427

MarkerSeek score 66.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 91
pi: 0.0427
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAGAAGCGGCCATAAACTG	CCAAAAGCAGACCTACGTCA	50.0	50.0	58.0	57.8	1401-2260 bp	1.0	87.5
ndhC-trnV-UAC_p2	GCGTCACCCATTGGTTCTAT	GCAGAATGAGGATAAGGGCC	50.0	55.0	58.0	58.1	1160-1176 bp	0.157895	46.9
ndhC-trnV-UAC_p3	TTTTCCGGCCCTTTGCTAAT	GCAGAATGAGGATAAGGGCC	45.0	55.0	58.1	58.1	1118-1134 bp	0.157895	46.6
ndhC-trnV-UAC_p4	AGAGAAGCGGCCATAAACTG	GCAGAATGAGGATAAGGGCC	50.0	55.0	58.0	58.1	1536-1552 bp	0.157895	46.5
ndhC-trnV-UAC_p5	CTGAAGTTTGGGTTGTCCGA	GCAGAATGAGGATAAGGGCC	50.0	55.0	58.0	58.1	1470-1486 bp	0.157895	46.5