Hotspot

trnT-UGU-trnL-UAA

LSC · 47500-47969 · 470 bp

Back to Garcinia

Pi 0.027

MarkerSeek score 65.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 42
pi: 0.027
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	511-540 bp	1.0	88.1
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	GCGTCTACCTATTTCGCCAT	55.0	50.0	58.2	57.8	542-571 bp	1.0	87.6
trnT-UGU-trnL-UAA_p3	CGAGTAAAGCCAGCTATCGG	GCGTCTACCTATTTCGCCAT	55.0	50.0	58.2	57.8	561-590 bp	1.0	86.8
trnT-UGU-trnL-UAA_p4	CTAACCTCTGAGCTAAGCGG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.5	57.8	503-532 bp	1.0	85.3
trnT-UGU-trnL-UAA_p5	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	513-542 bp	1.0	85.0