Hotspot

psbZ-trnG-GCC

LSC · 37245-38147 · 903 bp

Back to Garcinia

Pi 0.0327

MarkerSeek score 66.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 98
pi: 0.0327
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GGCGGATTCGTGGTAGAATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	1422-1522 bp	1.0	90.0
psbZ-trnG-GCC_p2	GGCGGATTCGTGGTAGAATT	TGGAAAGTTACAAGCCCCAC	50.0	50.0	58.1	58.0	1811-1903 bp	0.736842	77.8
psbZ-trnG-GCC_p3	CAAGACCGGAGCTATCAACC	TGGAAAGTTACAAGCCCCAC	55.0	50.0	58.1	58.0	2093-2198 bp	0.736842	77.7
psbZ-trnG-GCC_p4	TGGCCGGTTGTGAAGTAAAA	TGGAAAGTTACAAGCCCCAC	45.0	50.0	57.9	58.0	1963-2005 bp	0.157895	46.2
psbZ-trnG-GCC_p5	TGGCCGGTTGTGAAGTAAAA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.9	58.0	1582-1624 bp	0.157895	46.0