Hotspot

atpH-atpI

LSC · 14714-15976 · 1263 bp

Back to Garcinia

Pi 0.0266

MarkerSeek score 70.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1263
variable_sites: 116
pi: 0.0266
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCATGGAAGGGCATCATTGA	45.0	50.0	58.0	57.9	1275-1336 bp	1.0	90.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TCCATGGAAGGGCATCATTG	45.0	50.0	58.0	57.9	1276-1337 bp	1.0	90.2
atpH-atpI_p3	AGCGGCAGAAATCAATGGAT	CCATGGAAGGGCATCATTGA	45.0	50.0	57.9	57.9	1267-1328 bp	1.0	90.1
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	TCCATGGAAGGGCATCATTG	45.0	50.0	57.9	57.9	1268-1329 bp	1.0	90.1
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTGCAACTTTAGCTGCTGC	45.0	45.0	58.0	57.9	1309-1370 bp	1.0	89.6