Hotspot

trnS-GCU-trnG-UCC

LSC · 8242-9266 · 1025 bp

Back to Garcinia

Pi 0.0255

MarkerSeek score 66.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 90
pi: 0.0255
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTAGTCCACTCAGCCACC	TACCACTAAACTATACCCGC	55.0	45.0	57.9	54.0	1040-1189 bp	1.0	57.8
trnS-GCU-trnG-UCC_p2	CTTTAGTCCACTCAGCCACC	TTACCACTAAACTATACCCGC	55.0	42.9	57.9	54.9	1041-1190 bp	1.0	56.7
trnS-GCU-trnG-UCC_p3	TTTAGTCCACTCAGCCACCT	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	1039-1188 bp	1.0	56.4
trnS-GCU-trnG-UCC_p4	TTTAGTCCACTCAGCCACCT	TTACCACTAAACTATACCCGC	50.0	42.9	58.3	54.9	1040-1189 bp	1.0	55.3
trnS-GCU-trnG-UCC_p5	CTTTAGTCCACTCAGCCACC	TTTACCACTAAACTATACCCGC	55.0	40.9	57.9	55.6	1042-1191 bp	1.0	54.9