Hotspot

atpF

LSC · 26496-27762 · 1267 bp

Back to Gamochaeta

Pi 0.00632

MarkerSeek score 62.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1267
variable_sites: 8
pi: 0.00632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACAAAAAGAACTCTGTTCGA	ATTTCGTCGGCTTGAATGGT	35.0	45.0	53.0	58.2	1400-1401 bp	1.0	40.5
atpF_p2	ACAAAAAGAACTCTGTTCGA	GTCGGCTTGAATGGTTACCA	35.0	50.0	53.0	58.2	1395-1396 bp	1.0	40.5
atpF_p3	ACAAAAAGAACTCTGTTCGA	TCGTCGGCTTGAATGGTTAC	35.0	50.0	53.0	58.3	1397-1398 bp	1.0	40.5
atpF_p4	ACAAAAAGAACTCTGTTCGA	TTTCGTCGGCTTGAATGGTT	35.0	45.0	53.0	58.4	1399-1400 bp	1.0	40.5
atpF_p5	ACAAAAAGAACTCTGTTCGA	TTGCTCAATACGTTCACGGA	35.0	45.0	53.0	57.6	1431-1432 bp	1.0	40.5