Hotspot

trnL-UAG-rpl32

SSC · 122542-123201 · 660 bp

Back to Gamochaeta

Pi 0.00926
MarkerSeek score 65.80
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
660
variable_sites
6
pi
0.00926
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 778-837 bp 1.0 80.6
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 779-838 bp 1.0 80.6
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 789-848 bp 1.0 80.5
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 790-849 bp 1.0 80.5
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 57.9 57.9 788-847 bp 1.0 79.7