Hotspot

trnG-GCC-trnfM-CAU

LSC · 35803-36025 · 223 bp

Back to Gamochaeta

Pi 0.0314

MarkerSeek score 70.50

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 223
variable_sites: 7
pi: 0.0314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GAACGAAACAAAAAGCGGGT	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	57.8	58.0	378-393 bp	1.0	79.7
trnG-GCC-trnfM-CAU_p2	GAACGAAACAAAAAGCGGGT	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.8	58.0	342-357 bp	1.0	79.7
trnG-GCC-trnfM-CAU_p3	GAACGAAACAAAAAGCGGGT	GTCACGGGTTCAAATCCTGT	45.0	50.0	57.8	58.1	336-351 bp	1.0	79.2
trnG-GCC-trnfM-CAU_p4	GAACGAAACAAAAAGCGGGT	TCACGGGTTCAAATCCTGTC	45.0	50.0	57.8	57.8	335-350 bp	1.0	78.6
trnG-GCC-trnfM-CAU_p5	GAACGAAACAAAAAGCGGGT	TGAGGTCACGGGTTCAAATC	45.0	50.0	57.8	57.8	340-355 bp	1.0	78.6