Hotspot

trnT-GGU-psbD

LSC · 30877-32172 · 1296 bp

Back to Gamochaeta

Pi 0.00543

MarkerSeek score 62.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1296
variable_sites: 7
pi: 0.00543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGACCCGTTCAGGAATTCA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1477-1483 bp	1.0	79.3
trnT-GGU-psbD_p2	CAGTGACCCGTTCAGGAATT	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1479-1485 bp	1.0	79.2
trnT-GGU-psbD_p3	CGTAAGCCCTCGGTTCAAAT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1413-1419 bp	1.0	78.6
trnT-GGU-psbD_p4	TACAGTGACCCGTTCAGGAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1481-1487 bp	1.0	78.4
trnT-GGU-psbD_p5	TAAGCCCTCGGTTCAAATCC	GAAACGGTCCCTACGTAACC	50.0	55.0	57.6	58.0	1411-1417 bp	1.0	77.5