Hotspot

petN-psbM

LSC · 9901-10457 · 557 bp

Back to Gamochaeta

Pi 0.00372

MarkerSeek score 63.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 2
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.2	55.6	703-719 bp	1.0	61.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.2	56.4	703-719 bp	1.0	60.2
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	50.0	40.0	57.1	55.6	704-720 bp	1.0	55.9
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.9	55.6	695-711 bp	1.0	55.9
petN-psbM_p5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	47.6	40.0	58.0	55.6	689-705 bp	1.0	55.1