Hotspot

psbK-psbI

LSC · 7880-8294 · 415 bp

Back to Gamochaeta

Pi 0.00723

MarkerSeek score 59.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 3
pi: 0.00723
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	552 bp	1.0	72.5
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	549 bp	1.0	70.5
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	550 bp	1.0	70.5
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	551 bp	1.0	70.5
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCAGGATTACG	50.0	47.6	57.9	57.8	553 bp	1.0	70.2