Hotspot

rps16-trnQ-UUG

LSC · 6278-7270 · 993 bp

Back to Gamochaeta

Pi 0.00705

MarkerSeek score 52.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 7
pi: 0.00705
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1066-1080 bp	1.0	78.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1070-1084 bp	1.0	74.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1096-1110 bp	1.0	74.0
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1058-1072 bp	1.0	73.6
rps16-trnQ-UUG_p5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	1065-1079 bp	1.0	73.0