Hotspot

ycf1

SSC · 122501-127705 · 5205 bp

Back to Gamocarpha

Pi 0.00615

MarkerSeek score 42.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5205
variable_sites: 32
pi: 0.00615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTGTTGTGACTCGCTGATT	CTTCCCTATCCGAATTTCCGA	45.0	47.6	57.5	57.3	199 bp	1.0	64.2
ycf1_p2	AGTGTTGTGACTCGCTGATT	TCTTCCCTATCCGAATTTCCG	45.0	47.6	57.5	57.3	200 bp	1.0	64.2
ycf1_p3	ACACTACGATTTGGTCTCGG	CTTCCCTATCCGAATTTCCGA	50.0	47.6	57.4	57.3	229 bp	1.0	62.9
ycf1_p4	CACTACGATTTGGTCTCGGT	CTTCCCTATCCGAATTTCCGA	50.0	47.6	57.4	57.3	228 bp	1.0	62.9
ycf1_p5	CACTACGATTTGGTCTCGGT	TCTTCCCTATCCGAATTTCCG	50.0	47.6	57.4	57.3	229 bp	1.0	62.9