Hotspot

rpoA

LSC · 77593-78606 · 1014 bp

Back to Gamocarpha

Pi 0.0069

MarkerSeek score 60.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 7
pi: 0.0069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCAAAACCCATTTTCGGCCT	CATAATGGCTGTAGACCCCC	45.0	55.0	58.3	57.4	1189-1190 bp	1.0	73.6
rpoA_p2	TTCAAAACCCATTTTCGGCC	CATAATGGCTGTAGACCCCC	45.0	55.0	57.5	57.4	1190-1191 bp	1.0	71.6
rpoA_p3	CAAAACCCATTTTCGGCCTT	CATAATGGCTGTAGACCCCC	45.0	55.0	57.5	57.4	1188-1189 bp	1.0	71.6
rpoA_p4	TCAAAACCCATTTTCGGCCT	ATAATGGCTGTAGACCCCCG	45.0	55.0	58.3	58.9	1188-1189 bp	1.0	70.6
rpoA_p5	ACCCATTTTCGGCCTTTTTC	CATAATGGCTGTAGACCCCC	45.0	55.0	57.2	57.4	1184-1185 bp	1.0	69.3