Hotspot

rpl16-rps3

LSC · 82301-82451 · 151 bp

Back to Gamocarpha

Pi 0.0199

MarkerSeek score 63.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 3
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	GAGGGTAGGGTTCCACTACA	45.0	55.0	57.6	57.8	288-297 bp	1.0	75.7
rpl16-rps3_p2	AAACCAACGAGTCACACACT	ATCAGAGAGGGTAGGGTTCC	45.0	55.0	57.6	57.5	294-303 bp	1.0	73.8
rpl16-rps3_p3	AAACCAACGAGTCACACACT	GGATCAGAGAGGGTAGGGTT	45.0	55.0	57.6	57.5	296-305 bp	1.0	73.8
rpl16-rps3_p4	AAACCAACGAGTCACACACT	AGAGGGTAGGGTTCCACTAC	45.0	55.0	57.6	57.5	289-298 bp	1.0	73.3
rpl16-rps3_p5	AAACCAACGAGTCACACACT	TGGATCAGAGAGGGTAGGGT	45.0	55.0	57.6	58.7	297-306 bp	1.0	72.2