Hotspot

ndhC-trnV-UAC

LSC · 50776-51676 · 901 bp

Back to Gamocarpha

Pi 0.00723

MarkerSeek score 63.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 6
pi: 0.00723
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCTACTCGGGGCTAAAACTC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.1	57.8	980-1039 bp	1.0	82.4
ndhC-trnV-UAC_p2	TTGCTACTCGGGGCTAAAAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	982-1041 bp	1.0	82.1
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCCGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	973-1032 bp	1.0	81.8
ndhC-trnV-UAC_p4	TTCTCCGGTCCTTTGCTACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	994-1053 bp	1.0	80.0
ndhC-trnV-UAC_p5	CTTTGCTACTCGGGGCTAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	984-1043 bp	1.0	79.7