Hotspot

rpoB-trnC-GCA

LSC · 27362-28668 · 1307 bp

Back to Gamocarpha

Pi 0.00468

MarkerSeek score 62.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1307
variable_sites: 5
pi: 0.00468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCTATCCCGGAGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.5	58.1	1238-1391 bp	1.0	79.9
rpoB-trnC-GCA_p2	CCTCATTTCTATCCCGGAGC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.5	58.4	1220-1373 bp	1.0	77.8
rpoB-trnC-GCA_p3	TCCCTCATTTCTATCCCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.2	58.1	1240-1393 bp	1.0	77.3
rpoB-trnC-GCA_p4	TTCCCTCATTTCTATCCCGGA	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.9	58.1	1241-1394 bp	1.0	75.2
rpoB-trnC-GCA_p5	TCCCTCATTTCTATCCCGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.2	58.4	1222-1375 bp	1.0	75.2