Hotspot

trnK-UUU-rps16

LSC · 4339-5173 · 835 bp

Back to Gamocarpha

Pi 0.00481
MarkerSeek score 62.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
835
variable_sites
4
pi
0.00481
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.0 58.3 1012-1015 bp 1.0 77.8
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1029-1032 bp 1.0 77.5
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1028-1031 bp 1.0 77.5
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 968-971 bp 1.0 76.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 967-970 bp 1.0 76.8