Hotspot

ycf1

SSC · 128205-133865 · 5661 bp

Back to Gambeya

Pi 0.003

MarkerSeek score 44.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5661
variable_sites: 33
pi: 0.003
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAGCAAGCGGAATGGTATC	GTTCTATGCGAGCCCAAAGA	50.0	50.0	58.0	58.0	307 bp	1.0	80.7
ycf1_p2	TGAGCAAGCGGAATGGTATC	TTCTATGCGAGCCCAAAGAC	50.0	50.0	58.0	58.0	306 bp	1.0	80.7
ycf1_p3	TCTGTGCCAAGGTTTCAGAC	GTTCTATGCGAGCCCAAAGA	50.0	50.0	58.0	58.0	578 bp	1.0	80.5
ycf1_p4	TCTGTGCCAAGGTTTCAGAC	TCAAAGGTTCTATGCGAGCC	50.0	50.0	58.0	58.0	584 bp	1.0	80.5
ycf1_p5	TCTGTGCCAAGGTTTCAGAC	TTCTATGCGAGCCCAAAGAC	50.0	50.0	58.0	58.0	577 bp	1.0	80.5