Hotspot

ndhD

SSC · 119865-121376 · 1512 bp

Back to Gambeya

Pi 0.00287

MarkerSeek score 37.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1512
variable_sites: 8
pi: 0.00287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGACCGTATTCGCGATTT	AACCCGTGCTCGAGAATAAC	45.0	50.0	58.0	58.0	1956-1959 bp	1.0	80.8
ndhD_p2	TTGGACCGTATTCGCGATTT	TGTCGGTTGTAAGAGATGCG	45.0	50.0	58.0	58.0	2114-2117 bp	1.0	80.7
ndhD_p3	TTGGACCGTATTCGCGATTT	AAAGTATCTTGGCCCTCCCT	45.0	50.0	58.0	58.0	2410-2413 bp	1.0	80.6
ndhD_p4	TTGGACCGTATTCGCGATTT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	2137-2140 bp	1.0	80.6
ndhD_p5	TTGGACCGTATTCGCGATTT	GGGACGGGTGTAAAGCTAAG	45.0	55.0	58.0	58.0	2166-2169 bp	1.0	80.6