Hotspot

ndhA

SSC · 124049-126268 · 2220 bp

Back to Gambeya

Pi 0.00489

MarkerSeek score 51.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 21
pi: 0.00489
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGATGTTTGAGACAAGTCGTGA	CCGGGTTTTGTCAATTTGCA	40.9	45.0	57.9	57.8	2371-2377 bp	1.0	62.7
ndhA_p2	GTGATGTTTGAGACAAGTCGTG	CCGGGTTTTGTCAATTTGCA	45.5	45.0	57.9	57.8	2372-2378 bp	1.0	62.2
ndhA_p3	TGTGATGTTTGAGACAAGTCGT	CCGGGTTTTGTCAATTTGCA	40.9	45.0	58.2	57.8	2373-2379 bp	1.0	61.5
ndhA_p4	TGATGTTTGAGACAAGTCGTG	CCGGGTTTTGTCAATTTGCA	42.9	45.0	56.5	57.8	2371-2377 bp	1.0	59.2
ndhA_p5	TGTGATGTTTGAGACAAGTCG	CCGGGTTTTGTCAATTTGCA	42.9	45.0	56.5	57.8	2373-2379 bp	1.0	59.2