Hotspot

rps15-ycf1

SSC · 127816-128204 · 389 bp

Back to Gambeya

Pi 0.0146

MarkerSeek score 61.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 389
variable_sites: 10
pi: 0.0146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGACCCTCGGTTTTC	TGGCAGTCGTTTCAGTATGT	50.0	45.0	57.8	57.2	501-503 bp	1.0	73.3
rps15-ycf1_p2	CAACAGACCCTCGGTTTTCT	TGGCAGTCGTTTCAGTATGT	50.0	45.0	57.7	57.2	500-502 bp	1.0	73.2
rps15-ycf1_p3	TCAACAGACCCTCGGTTTTC	TGGCAGTCGTTTCAGTATGTT	50.0	42.9	57.8	57.9	501-503 bp	1.0	71.0
rps15-ycf1_p4	CAACAGACCCTCGGTTTTCT	TGGCAGTCGTTTCAGTATGTT	50.0	42.9	57.7	57.9	500-502 bp	1.0	70.9
rps15-ycf1_p5	ACAGACCCTCGGTTTTCTTC	TGGCAGTCGTTTCAGTATGT	50.0	45.0	57.5	57.2	498-500 bp	1.0	70.9