Hotspot

ccsA-ndhD

SSC · 119669-119864 · 196 bp

Back to Gambeya

Pi 0.0255

MarkerSeek score 69.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 196
variable_sites: 8
pi: 0.0255
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGTTACTTTGGGGTCA	ACCCCGATTTCGTTCTTTCA	40.9	45.0	56.8	57.4	353-355 bp	1.0	52.4
ccsA-ndhD_p2	ACTTTGGGGTCAATCTTTTAGGA	ACCCCGATTTCGTTCTTTCA	39.1	45.0	57.7	57.4	343-345 bp	1.0	51.5
ccsA-ndhD_p3	TGGATATGTTACTTTGGGGTCAA	ACCCCGATTTCGTTCTTTCA	39.1	45.0	57.5	57.4	353-355 bp	1.0	49.9
ccsA-ndhD_p4	TTGGATATGTTACTTTGGGGTCA	ACCCCGATTTCGTTCTTTCA	39.1	45.0	57.5	57.4	354-356 bp	1.0	49.9
ccsA-ndhD_p5	TGGATATGTTACTTTGGGGTCA	ACCCCGATTTCGTTCTTTCAT	40.9	42.9	56.8	57.9	353-355 bp	1.0	48.3