Hotspot

petD-rpoA

LSC · 81380-81629 · 250 bp

Back to Gambeya

Pi 0.0147

MarkerSeek score 56.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 250
variable_sites: 6
pi: 0.0147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TGGGTATTGGCGCAACATTA	TTGGGCATTCTAGAAAAGCA	45.0	40.0	57.9	55.2	325-337 bp	1.0	60.1
petD-rpoA_p2	CCAGTAGCAACAACCGTCTT	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	374-386 bp	1.0	59.8
petD-rpoA_p3	TGGGTATTGGCGCAACATTA	TGGGCATTCTAGAAAAGCAT	45.0	40.0	57.9	55.0	324-336 bp	1.0	58.2
petD-rpoA_p4	CCAGTAGCAACAACCGTCTT	TGGGCATTCTAGAAAAGCAT	50.0	40.0	58.1	55.0	373-385 bp	1.0	57.8
petD-rpoA_p5	TTTGATTGGTACGGCAGTGG	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.5	55.2	354-366 bp	1.0	57.1