Hotspot

petA-psbJ

LSC · 66140-67225 · 1086 bp

Back to Gambeya

Pi 0.0054

MarkerSeek score 58.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 11
pi: 0.0054
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1183-1196 bp	1.0	77.5
petA-psbJ_p2	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1184-1197 bp	1.0	75.1
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	TGGTTCATATTCCGGATTGGG	45.0	47.6	57.8	57.8	1191-1204 bp	1.0	70.6
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	GGTTCATATTCCGGATTGGGT	45.0	47.6	57.8	57.8	1190-1203 bp	1.0	70.6
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	1133-1146 bp	1.0	70.0