Hotspot

rbcL-accD

LSC · 59285-59876 · 592 bp

Back to Gambeya

Pi 0.00169

MarkerSeek score 58.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	GGATCTTCACTTTCGCTGGT	50.0	50.0	57.9	57.9	763-779 bp	1.0	79.3
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	GGATCTTCACTTTCGCTGGT	55.0	50.0	57.7	57.9	779-795 bp	1.0	77.9
rbcL-accD_p3	CTGCTGCTTGTGAGGTATGG	GGATCTTCACTTTCGCTGGT	55.0	50.0	58.6	57.9	765-781 bp	1.0	75.2
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	ACCCACACCTGTGTTCTAAC	50.0	50.0	57.9	57.4	705-721 bp	1.0	75.1
rbcL-accD_p5	CTTGCTCGTGAGGGTAATGA	GGATCTTCACTTTCGCTGGT	50.0	50.0	57.3	57.9	823-839 bp	1.0	74.8