Hotspot

atpB-rbcL

LSC · 57062-57856 · 795 bp

Back to Gambeya

Pi 0.00672

MarkerSeek score 57.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 9
pi: 0.00672
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGAATGATACGCCCCAGG	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	906-922 bp	1.0	79.4
atpB-rbcL_p2	GAATGATACGCCCCAGGTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	903-919 bp	1.0	79.4
atpB-rbcL_p3	TTTGAATGATACGCCCCAGG	TAACACCCGCTTTGAATCCA	50.0	45.0	57.9	57.4	907-923 bp	1.0	77.2
atpB-rbcL_p4	GAATGATACGCCCCAGGTTT	TAACACCCGCTTTGAATCCA	50.0	45.0	57.9	57.4	904-920 bp	1.0	77.2
atpB-rbcL_p5	TTTGAATGATACGCCCCAGG	CCCGCTTTGAATCCAACACT	50.0	50.0	57.9	58.8	902-918 bp	1.0	75.8