Hotspot

ycf3-trnS-GCU

LSC · 46832-47687 · 856 bp

Back to Gambeya

Pi 0.00371

MarkerSeek score 62.20

Primer available yes

Label ycf3-trnS-GCU

Feature Metadata

label_name: ycf3-trnS-GCU
feature_type: igs
region: LSC
length_bp: 856
variable_sites: 6
pi: 0.00371
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GCU_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	960-966 bp	1.0	71.9
ycf3-trnS-GCU_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	910-916 bp	1.0	71.6
ycf3-trnS-GCU_p3	ATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.6	58.2	959-965 bp	1.0	68.8
ycf3-trnS-GCU_p4	TTTATCCGCGATCTAGGCAT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.6	58.2	958-964 bp	1.0	68.8
ycf3-trnS-GCU_p5	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTG	47.6	50.0	58.0	58.5	912-918 bp	1.0	68.8