Hotspot

trnH-GUG-psbA

LSC · 95-623 · 529 bp

Back to Gambeya

Pi 0.0107

MarkerSeek score 57.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 529
variable_sites: 11
pi: 0.0107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTAGTTATGGGCGAACGACG	CCTCTAGACCTCGCTGCTAT	50.0	55.0	58.1	58.1	657-689 bp	1.0	80.7
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTCGCTGCTAT	50.0	55.0	57.9	58.1	602-634 bp	1.0	80.5
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTCGCTGCTAT	50.0	55.0	58.2	58.1	603-635 bp	1.0	80.3
trnH-GUG-psbA_p4	AATCCACTTGGCTACATCCG	CCTCTAGACCTCGCTGCTAT	50.0	55.0	57.7	58.1	595-627 bp	1.0	78.9
trnH-GUG-psbA_p5	TTAGTTATGGGCGAACGACG	AACTTCCCTCTAGACCTCGC	50.0	55.0	58.1	58.5	663-695 bp	1.0	77.2