Hotspot

ycf3

LSC · 42850-44954 · 2105 bp

Pi 0.0241

MarkerSeek score 66.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2105
variable_sites: 119
pi: 0.0241
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	CAAACCAAATTGCGCCAGAA	GGAAATCGAGTCGGGTTCAA	45.0	50.0	58.1	57.9	2606-2806 bp	1.0	86.8
ycf3_p2	CAAACCAAATTGCGCCAGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	58.1	58.1	2781-2988 bp	1.0	86.8
ycf3_p3	AAACCAAATTGCGCCAGAAG	GGAAATCGAGTCGGGTTCAA	45.0	50.0	57.8	57.9	2605-2805 bp	0.857143	80.5
ycf3_p4	ATTCGTGGCAAGTGTTCGTA	GGAAATCGAGTCGGGTTCAA	45.0	50.0	57.8	57.9	2854-2943 bp	0.714286	73.5
ycf3_p5	TTCGTGGCAAGTGTTCGTAT	GGAAATCGAGTCGGGTTCAA	45.0	50.0	57.8	57.9	2853-2942 bp	0.714286	73.5