Hotspot

infa-rps8

LSC · 79299-79585 · 287 bp

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Pi 0.0893
MarkerSeek score 75.40
Primer available yes
Label infa-rps8

Feature Metadata

label_name
infa-rps8
feature_type
igs
region
LSC
length_bp
287
variable_sites
50
pi
0.0893
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.95
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infa-rps8_p1 GCACCCAAGGAGTCTTCAAA GCGGGATGGGGATTGTAATT 50.0 50.0 58.0 58.0 520-692 bp 1.0 91.8
infa-rps8_p2 GCACCCAAGGAGTCTTCAAA TTCCTCGAATTTTAGGCGGG 50.0 50.0 58.0 58.0 535-707 bp 1.0 91.6
infa-rps8_p3 TCCTTTTGGTATTCTCCGCG GCGGGATGGGGATTGTAATT 50.0 50.0 58.0 58.0 926-1084 bp 1.0 90.9
infa-rps8_p4 TCCTTTTGGTATTCTCCGCG TTCCTCGAATTTTAGGCGGG 50.0 50.0 58.0 58.0 941-1099 bp 1.0 90.6
infa-rps8_p5 GCGTCTCCTGGTTGAATCAT GCGGGATGGGGATTGTAATT 50.0 50.0 58.0 58.0 485-621 bp 0.714286 80.0