Hotspot

psaJ-rpl33

LSC · 66614-67092 · 479 bp

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Pi 0.0459
MarkerSeek score 81.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
479
variable_sites
52
pi
0.0459
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 CGAACTGAACTAAGAGCGCT ACGCCTACGAAAAGATTGCT 50.0 45.0 58.0 57.9 1587-1675 bp 1.0 87.1
psaJ-rpl33_p2 CGAACTGAACTAAGAGCGCT TCGTACACCTTTACCCTCGT 50.0 50.0 58.0 58.1 1225-1289 bp 0.571429 68.3
psaJ-rpl33_p3 CCCCCGTTACTGCTACTTTC TCGTACACCTTTACCCTCGT 55.0 50.0 58.0 58.1 1101-1165 bp 0.571429 68.2
psaJ-rpl33_p4 CGAACTGAACTAAGAGCGCT GCCGTTACTCGTACACCTTT 50.0 50.0 58.0 57.9 1254 bp 0.142857 45.0
psaJ-rpl33_p5 CCCCCGTTACTGCTACTTTC GCCGTTACTCGTACACCTTT 55.0 50.0 58.0 57.9 1129 bp 0.142857 45.0