Hotspot

trnT-GGU-psbD

LSC · 31638-33067 · 1430 bp

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Pi 0.0503
MarkerSeek score 85.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1430
variable_sites
163
pi
0.0503
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1470-1534 bp 1.0 52.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1473-1537 bp 1.0 52.0
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1447-1511 bp 1.0 52.0
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1469-1533 bp 1.0 52.0
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.1 52.8 1472-1536 bp 1.0 52.0