Hotspot

trnE-UUC-trnT-GGU

LSC · 30682-31565 · 884 bp

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Pi 0.0759
MarkerSeek score 86.40
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
884
variable_sites
159
pi
0.0759
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 966-1114 bp 1.0 89.7
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 945-1093 bp 1.0 89.2
trnE-UUC-trnT-GGU_p3 TGTCCTGAACCACTAGACGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.7 58.1 942-1090 bp 1.0 89.1
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA CCGATGACTTACGCCTTACC 50.0 55.0 57.7 58.2 963-1111 bp 1.0 88.5
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA TTGAACCGATGACTTACGCC 50.0 50.0 57.7 58.3 968-1116 bp 1.0 87.5