Hotspot

petN-psbM

LSC · 28895-29604 · 710 bp

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Pi 0.0416
MarkerSeek score 81.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
710
variable_sites
69
pi
0.0416
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTTCGTGATTCTCTTGGCC GTAGAGGGGTGGTCTGATGA 50.0 55.0 57.9 57.8 1701-1782 bp 0.714286 73.0
petN-psbM_p2 ACTTCGTGATTCTCTTGGCC AGTAGAGGGGTGGTCTGATG 50.0 55.0 57.9 57.8 1702-1783 bp 0.714286 73.0
petN-psbM_p3 ACTTCGTGATTCTCTTGGCC TGGCAGGATATAAGGGCGTA 50.0 50.0 57.9 58.0 1850-1882 bp 0.428571 60.7
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT TGGCAGGATATAAGGGCGTA 50.0 50.0 58.2 58.0 1340-1379 bp 0.428571 59.9
petN-psbM_p5 ACTTCGTGATTCTCTTGGCC GAGCCGGGGGTAAAATCTTT 50.0 50.0 57.9 57.9 1922 bp 0.142857 43.8