Hotspot

trnC-GCA-petN

LSC · 28123-28804 · 682 bp

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Pi 0.0341
MarkerSeek score 80.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
682
variable_sites
58
pi
0.0341
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 745-762 bp 1.0 87.7
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 763-780 bp 1.0 85.3
trnC-GCA-petN_p3 GGTGTCGCCTGATCAACAAA AGCCCAAGCGAGACTTACTA 50.0 50.0 58.8 58.2 726-743 bp 1.0 82.5
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG GCCCAAGCGAGACTTACTAT 50.0 50.0 58.1 57.1 744-761 bp 1.0 81.8
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAG 50.0 50.0 58.1 58.9 753-770 bp 1.0 81.6